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Frequently Asked Questions (FAQ)

• Can you measure proteins with SpectroCuvette™ One?
• How is refractive index different from absorbance, which is conventionally measured with a spectrophotometer?
• How to do 0.5 uL measurements with SpectroCuvette™ One
• How to do 3 mL measurements with SpectroCuvette™ One
• Is it possible to do kinetics with SpectroCuvette™ One?
• What can I use the refractive index for?
• What do I need in my lab to get started with SpectroCuvette™ One?
• What do you measure with SpectroCuvette™ One?
• What is an appropriate reference solution when using SpectroCuvette™ One?
• What is full volume use with SpectroCuvette™ One?

  For full volume use of SpectroCuvette™ One, we recommend filling cuvette with 3 mL of liquid sample

• What is low volume use with SpectroCuvette™ One?

  For low volume use, we recommend 0.5 uL sample volume using the glass insert slides shipped with every SpectroCuvette™ One package

• What path lengths are used to make measurements, and is the user required to make any calculations relevant to the path length when measuring with SpectroCuvette™ One?
• What types of samples can be measured with SpectroCuvette™ One?
• Which sample volumes can be used to make measurements with SpectroCuvette™ One?
• Which UV-Vis spectrophotometers are supported with SpectroCuvette™ One?

  All UV-Vis spectrophotometers with a 12 mm x 12 mm cuvette footprint for 10 mm optical path length and capable of exporting spectra as data files are supported by SpectroCuvette™ One.

• Will the sample size of the volume affect the concentration when using SpectroCuvette™ One?