UV-Vis library
Knowledge base
Guides, application notes and instrument documentation for SpectroCuvette™, SpectroLink™ and SpectroWorks™.
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Start with the instrument you use
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SpectroShell™
SpectroShell™ is an instrument casing designed to improve repeatability and precision for spectrophotometers without a cuvette holder system.
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SpectroCuvette™ One
SpectroCuvette™ One measures surface refractive index and absorbance for accurate and fast liquid analysis for both 3 mL and 0.5 uL volumes.
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SpectroLink™
Electronic device that connects to spectrophotometers for seamless instrument control, data management and analysis.
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SpectroWorks™
SpectroWorks™ is a modern software platform for UV-Vis spectrophotometry, particle size analysis and label-free analysis.
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Spectrophotometry fundamentals
Learn everything that you need to know about modern spectrophotometry, what it can be used for and how it can be applied in various fields.
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SpectroCuvette™ S
Semi-disposable cuvette consumable with an embedded optical chip for particle size analysis, cell/bacteria size and concentration analysis.
Selected documentation
Guides from the UV-Vis knowledge base
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Application Note AN-6-101: Particle size analysis of polystyrene beads with UV-VIS spectrophotometer and SpectroCuvette™ S
The SpectroCuvette™ S is a low-cost alternative to other PSA techniques such as Dynamic Light Scattering (DLS) or Laser Diffraction here illustrated with polystyrene latex beads.
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Validation report: Testing and validation of SpectroCuvette™ S, SpectroWorks™ together with UV-Vis spectrophotometer
The original report by Particle Analytical ApS including appendices can be downloaded here (pdf). Report written by Wenbo Wang, QC, and approved by Søren Lund Kristensen, QA at Particle Analytical ApS - Agern Alle 3, 2970 Hørsholm, Denmark. Date: 30-jul-2021 Table of…
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Webinar about SpectroCuvette™ S
Here you can watch the webinar “Achieve easy, fast and reliable particle/cell size analysis with SpectroCuvette™ S” and download the slides from the presentation.
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Application Note AN-1-102: Sugar content via °Brix index and refractometry with SpectroCuvette™ One and UV-Vis spectrophotometer
SpectroCuvette™ One uses existing spectrophotometers and the SpectroWorks™ software to accurately measure refractive index, °Brix index and sucrose concentrations. Results are not affected by impurities and have a wide refractive index range.
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Droplets and micro-volume sample applications for SpectroCuvette™ One
Micro-volume samples around 0.5 µL can be measured at low cost in a conventional UV-Vis spectrophotometer by dispensing a droplet and flattening it on the optical filter of SpectroCuvette™ One.
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White paper: Protein quantification using spectrophotometer, SpectroCuvette™ One and SpectroWorks™ compared to micro-volume system
Sangita Khatri¹, Thomas Tølbøl Sørensen¹, Arpita De¹, Emil Højlund-Nielsen¹, Jesper Uhd² ¹Alontia, Copenhagen, Hørmarken 2, DK-3520 Farum, Denmark ²Technical University of Denmark, Department of Chemistry, Kemitorvet 206, 2800 Kongens Lyngby, Denmark Published July 21, 2021 Download pdf here Keywords Protein, UV-Vis Spectrophotometer,…
Quick answers
Common questions
Can I use SpectroCuvette™ S more than once?
SpectroCuvette™ S: it is strongly recommended that each cuvette is only used once, as the product cannot be guaranteed to perform nominally after sample contact. However, there is a cleaning protocol that has been tested on SpectroCuvette™ One for reusability of the…
Can you measure proteins with SpectroCuvette™ One?
Yes, proteins can be quantified with SpectroCuvette™ One. Please refer to protein application page for details.
Can SpectroCuvette™ S be used for measuring particle sizes?
Yes, SpectroCuvette™ S can be used for measuring particle sizes.
How to do 0.5 uL measurements with SpectroCuvette™ One
The SpectroCuvette™ One allows for 0.5 uL measurements. The procedure is described in this application article.
Is it possible to do kinetics with SpectroCuvette™ S?
Yes, it is possible to follow experiments in real-time and measure kinetics.
Which UV-Vis spectrophotometers are supported by SpectroLink™?
Here you can find a list of spectrophotometers from Edinburgh Instruments, Ocean Insight (OceanOptics), VWR, and Shimadzu supported by SpectroLink™.